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JoVE Journal
Immunology and Infection

This content is Open Access.

Production and Characterization of Human Macrophages from Pluripotent Stem Cells
 

Production and Characterization of Human Macrophages from Pluripotent Stem Cells

Article doi: 10.3791/61038
April 16th, 2020

Summary April 16th, 2020

This protocol describes the robust generation of macrophages from human induced pluripotent stem cells, and methods for their subsequent characterization. Cell surface marker expression, gene expression, and functional assays are used to assess the phenotype and function of these iPSC-derived macrophages.

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