Name: Surgical Approach to Full Soft Tissue Face Allograft Procurement for Vascularized Composite Allotransplantation (Video) | JoVE Journal
Uploaded: 2025-12-30T14:33:00.000+00:00
Duration: 9 min 14 s
Description: Learn the surgical protocol for full soft tissue face allograft procurement using cervical and coronal dissection techniques. This standardized approach preserves vascular and neural structures for vascularized composite allotransplantation.

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5 minutes

The authors wish to express their sincere gratitude to the individuals who generously donated their bodies to science, thereby enabling anatomical research.

The Anatomy Laboratory of the Faculty of Medicine of Nice, France, generously provided the specimens and materials used in this study. The study was approved by the French National Ethics Committee (IRB approval No. IRB00014528_2025_36) and conducted in accordance with the principles of the Declaration of Helsinki.
1. Positioning and airway preparation
<ol>
	<li>Place the donor supine on the operating table.</li>
	<li>Perform a tracheotomy to secure the airway. 
	NOTE: Whenever possible, perform procurement under beating-heart conditions to minimize ischemic time. The facial allograft should be harvested prior to the retrieval of other solid organs to maintain tissue quality without compromising subsequent graft outcomes. Apply a cast mold to the donor's face (facial mask impression) before beginning procurement.</li>
	<li>Delineate the planned incisions, using a surgical marking pen: Coronal incision; Pretragal incision; Lateral cervical incision; Transverse incision at the level of the hyoid bone.</li>
	<li>Infiltrate with local anesthesia (lidocaine + epinephrine 2%) 
	​NOTE: Begin at the scalp and proceed inferiorly along the preauricular area. Extend the infiltration to a point approximately 5 cm below the mandibular angle, then continue anteriorly across the cervical region to join the contralateral side above the tracheotomy site.</li>
</ol>
<img alt="Autopsy study of facial muscles and neck posture; anatomical examination for medical education." class="xfigimg" src="/files/ftp_upload/69607/69607fig1.jpg" data-altupdatedat="1773047343000" data-alt="Figure 1"> 
Figure 1: Preoperative skin markings of the planned incisions. (A) Anterior view showing a transverse incision at the level of the hyoid bone. (B) Lateral view showing the lateral coronal incision, the preauricular incision and the lateral cervical incision along the sternocleidomastoid muscle contour. <a href="https://www.jove.com/files/ftp_upload/69607/69607fig1large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
2. Cervical dissection
<ol>
	<li>Make a cervical incision extending from the superior border of the clavicle to the earlobe, using a No. 10 or 15 scalpel blade.</li>
	<li>Raise the cervical skin flap in the subplatysmal plane using a fine-tip monopolar electrocautery set to approximately 20-30 °C and 80 W in coagulation mode and Adson tissue forceps.</li>
	<li>Dissect the neck area to identify the sternocleidomastoid muscle using strabismus scissors.</li>
	<li>Perform a circumferential dissection of the external jugular vein, which is ligated and divided using strabismus scissors.</li>
	<li>Retract the sternocleidomastoid muscle laterally using a Farabeuf retractor to gain access to the vascular pedicles. Free up this muscle laterally to expose the vessels.</li>
	<li>Dissect carefully using strabismus scissors to expose the internal jugular vein and subsequent thyro-linguofacial trunk and identify and preserve the facial vein. Control minor bleeding with low-intensity coagulation, using a standard monopolar electrocautery set to 10-20 W in coagulation mode, or a bipolar device set to 10-20 W to maintain precise hemostasis. 
	NOTE: Use low-intensity coagulation and maintain constant visualization of the carotid bifurcation to avoid thermal injury to the vagus and hypoglossal nerves.</li>
	<li>Dissect carefully using strabismus scissors to expose the common carotid artery to reveal the carotid bifurcation.</li>
	<li>Dissect the external carotid artery using strabismus scissors cranially to reveal all of the branches: ligate and divide the superior thyroid, ascending pharyngeal, occipital arteries, and lingual. Preserve the facial branch.</li>
	<li>Identify the posterior belly of the digastric muscle and stylohyoid muscle.</li>
	<li>Transect both muscles near their insertions to enhance surgical exposure using a fine-tip monopolar electrocautery set to approximately 30 °C in coagulation mode.</li>
	<li>Locate the hypoglossal nerve, and section it using a fine-tip monopolar electrocautery set to approximately 20-30 °C in coagulation mode proximally to obtain maximal length for potential nerve grafting.</li>
	<li>Identify, ligate, and divide the submandibular gland, duct, and vessels. Exclude the submandibular gland from the allograft. 
	NOTE: The submandibular gland is excluded to minimize postoperative salivary complications such as sialocele or infection, which are more frequent when the gland or its duct is included within the graft. This choice is consistent with clinical practice in face transplantation, where exclusion of salivary glands, particularly the submandibular gland, reduces bulk and improves contour definition21.</li>
	<li>Repeat the above cervical dissection steps on the opposite side.</li>
</ol>
<img alt="Human anatomy dissection, labeled nerve pathways and vessels, educational medical study." class="xfigimg" src="/files/ftp_upload/69607/69607fig2.jpg" data-altupdatedat="1773047343000" data-alt="Figure 2"> 
Figure 2: Cervical dissection and exposure of the neck structure at their origin. (1) Common carotid artery, (2) External carotid artery, (3) Occipital artery, (4) Posterior auricular artery, (5) Facial artery, (6) Lingual artery, (7) Superior thyroid artery, (8) Internal jugular vein, (9) Facial vein, (10) Vagus nerve, (11) Ansa cervicalis, (12) Hypoglossal nerve. <a href="https://www.jove.com/files/ftp_upload/69607/69607fig2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
3. Facial nerve dissection
<ol>
	<li>Perform the pretragal incision using a No. 10 or 15 scalpel blade to dissect a preauricular skin flap using strabismus scissors in the sub-superficial musculoaponeurotic system plane.</li>
	<li>Transect the external auditory canal using a fine-tip monopolar electrocautery set to approximately 30 °C in coagulation mode to expose the facial nerve at its origin (retro-auricular) 
	NOTE: Transection of the external auditory canal is commonly used in cadaveric or experimental protocols, as it provides a faster and more straightforward exposure. A retroauricular dissection of the main trunk of the facial nerve, using a parotidectomy-type approach, can also be performed while preserving the external auditory canal. In clinical face transplantation, this latter approach is systematically preferred to preserve the integrity of the auditory canal and external ear structures. Avoid excessive traction on the facial nerve trunk to prevent avulsion or transection at the stylomastoid foramen.</li>
	<li>Dissect using strabismus scissors and ligate using absorbable monofilament suture the maxillary artery (if not done previously).</li>
	<li>Liberate all of the anterior soft tissues en bloc - including the superficial temporal vessels, using strabismus scissors. 
	​NOTE: For clinical purpose, the exclusion of parotid and remaining salivary glands should be realized to minimize the formation of sialocele6. This involves a superficial parotidectomy with facial nerve dissection. In the context of cadaveric training, it may be faster to include the parotid gland within the graft to avoid potential facial nerve injury and time-consuming dissection.</li>
	<li>Isolate the facial nerve with a vessel loop and dissect it to obtain maximal length using strabismus scissors.</li>
	<li>Repeat the same dissection on the contralateral side.</li>
</ol>
<img alt="Anatomy dissection image showing cranial nerve pathways, labeled for educational study." class="xfigimg" src="/files/ftp_upload/69607/69607fig3.jpg" data-altupdatedat="1773047343000" data-alt="Figure 3"> 
Figure 3: Facial nerve dissection. (1) Facial nerve, (2) External carotid artery, (3) Superficial temporal artery. <a href="https://www.jove.com/files/ftp_upload/69607/69607fig3large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
4. Coronal approach for scalp dissection and periorbital management
<ol>
	<li>Make a coronal incision using a No. 10 or 15 scalpel blade and elevate the scalp flap in a sub-periosteal plane posterior to anterior, until the supraorbital nerve is encountered using a fine-tip monopolar electrocautery set to approximately 30 °C in coagulation mode. 
	NOTE: The temporalis muscle is excluded from the allograft.</li>
	<li>Ligate and divide the supraorbital and supratrochlear neurovascular contents as close as possible to the bony foramen, using absorbable monofilament suture and strabismus scissors.</li>
	<li>Locate the Levator Palpebrae Superioris (LPS) muscle with globe in situ and place a marking suture (Ethilon). 
	NOTE: At this stage, hydrodissection may be used to facilitate dissection and reduce trauma to adjacent structures.</li>
	<li>Proceed with the periorbital dissection using strabismus scissors in the subperiosteal plane to preserve the orbicularis oculi within the graft by dividing the upper eyelid horizontally.</li>
	<li>Perform circumferential transconjunctival incisions using a No. 10 or 15 scalpel blade to maximally preserve the superior and inferior tarsal plates, orbital septum, levator palpebrae superioris, and Müller's muscle.</li>
	<li>Isolate the lateral canthus and elevate it with a small segment of the lateral orbital rim using an osteotome, ensuring proper canthal support within the graft.</li>
	<li>Excise the lower eyelid while preserving the conjunctiva. Dissect through the inferior fornix using strabismus scissors, just inside the orbital rim, allowing preservation of the orbital septum, fat, and lower lid retractors.</li>
	<li>Identify and transect the infraorbital nerve near its foramen using a fine-tip monopolar electrocautery set to approximately 30 °C in coagulation mode, ensuring maximal nerve length for future neurorrhaphy.</li>
</ol>
<img alt="Surgical procedure showcasing facial anatomy with labeled sutures for educational study." class="xfigimg" src="/files/ftp_upload/69607/69607fig4.jpg" data-altupdatedat="1773047343000" data-alt="Figure 4"> 
Figure 4: Coronal dissection. (1) Supraorbital nerve, (2) Supratrochlear nerve. <a href="https://www.jove.com/files/ftp_upload/69607/69607fig4large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
5. Elevation of the facial flap and extension toward the midface
<ol>
	<li>Section the facial nerve using strabismus scissors at its exit from the stylomastoid foramen, shaving the masseter muscle to expose the mandibular surface</li>
	<li>Raise the flap anteriorly on top of the masseter and identify the buccal fat pad at its anterior border, using a fine-tip monopolar electrocautery set to approximately 30 °C in coagulation mode. Continue anterior dissection along the buccal fat pad (left in situ) until reaching and harvesting the buccal mucosa in full.</li>
	<li>Extend the dissection to the orbit and the zygoma.</li>
</ol>
6. Midface osteotomies
<ol>
	<li>Perform a subperiosteal dissection lateral to the nose using strabismus scissors, preserving the medial tendon canthal attachments.</li>
	<li>Perform a low-to-low lateral nasal osteotomy using a reciprocating saw and a curved osteotome, extending to the hard palate. 
	NOTE: Control instrument orientation to avoid injury to the infraorbital nerve.</li>
	<li>Extend the osteotomy laterally to include the zygomatic buttress and follow the zygomaticofrontal and sphenozygomatic sutures of the maxilla and zygoma.</li>
	<li>Divide the nasal septum longitudinally using strabismus scissors. Harvest the nose along with the skin, cartilage (alar, triangular, and most of the septum), nasal bones, and mucosa. 
	​NOTE: For advanced training or research applications, the harvest can be extended to include osseous components such as the maxilla or zygoma, using donor-specific cutting guides. In these cases, the facial artery remains the primary vascular pedicle, with possible contribution from the infraorbital and transverse facial arteries depending on the extent of bone involvement.</li>
</ol>
<img alt="Human anatomy dissection setup, cranial nerve identification, educational experiment." class="xfigimg" src="/files/ftp_upload/69607/69607fig5v2.jpg" data-altupdatedat="1773047343000" data-alt="Figure 5"> 
Figure 5: Midface dissection. (1) Infraorbital nerve. For demonstration purposes, the infraorbital nerve was preserved and not divided prior to nasal harvest. <a href="https://www.jove.com/files/ftp_upload/69607/69607fig5large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
7. Intraoral dissection and flap liberation
<ol>
	<li>Make a circumferential intraoral incision using a No. 10 or 15 scalpel blade, along the gingivobuccal mucosal junction.</li>
	<li>Proceed by incising both the superior (maxillary) and inferior (mandibular) gingival mucosa using the scalpel, following the dental arcades precisely.</li>
	<li>Incise the gingival mucosa along the mandibular line.</li>
	<li>Identify and divide the mental nerve close to its mental foramen, preserving as much length as possible for potential neurorrhaphy, using strabismus scissors.</li>
	<li>Cut the mucosa at the gingiva, on the dental part of the maxilla and mandible.</li>
	<li>Divide the mental nerve close to its foramen using strabismus scissors.</li>
	<li>Mobilize the cervical soft tissue planes medially and direct them toward the oral cavity using a fine-tip monopolar electrocautery set to approximately 30 °C in coagulation mode, dissect in the plane above the sternohyoid and superior omohyoid muscles, with or without inclusion of the anterior jugular vein.</li>
	<li>Elevate the graft en bloc, by liberating the allograft from all soft-tissue attachments using the monopolar electrocautery besides the bilateral vascular pedicles.</li>
	<li>Ligate the internal and external jugular veins and carotid arteries using absorbable monofilament as distally as possible.</li>
	<li>Detach the face entirely.</li>
</ol>
<img alt="Facial dissection skin flap technique; anatomical study; comparative analysis of two specimens." class="xfigimg" src="/files/ftp_upload/69607/69607fig6.jpg" data-altupdatedat="1773047343000" data-alt="Figure 6"> 
Figure 6: Final aspect of the harvested graft. The facial nerve is tagged with a blue suture on each side of the graft. (A) Internal view, soft tissue side. White arrow: orbital septum. Black arrow: superior gingival mucosa. Green arrow: inferior gingival mucosa. (B) External view, epidermal surface. <a href="https://www.jove.com/files/ftp_upload/69607/69607fig6large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
8. Packaging of the graft
<ol>
	<li>Cannulate the external carotid arteries with vascular cannulas secured by silk ties. 
	NOTE: Confirm hemostasis before perfusion and handle cannulas with care to prevent vessel wall tearing.</li>
	<li>Connect a cysto irrigation set to the stopcock and flush with the institution transplant preservation solution until clear venous effluent is observed. 
	NOTE: For graft perfusion and storage, preservation solutions such as University of Wisconsin (UW) at 4 °C or Histidine-Tryptophan-Ketoglutarate (HTK) supplemented with 5,000 units of unfractionated heparin may be used.</li>
	<li>Disconnect the Cysto Irrigation Set and close the stopcock.</li>
	<li>Wrap the graft in saline-moistened sterile towels, place it in a sealed sterile bag, and label. Enclose in a second sterile empty bag, then a third sterile bag containing ice water, in accordance with standard solid organ transplantation preservation strategies. In line with solid organ transplantation, the recommended target cold ischemia time for VCA grafts is ideally maintained under 4-6 h to optimize tissue viability. 
	NOTE: Following procurement, reconstructive measures are undertaken to ensure a respectful presentation of the donor. These typically include re-approximation of skin edges, placement of an acrylic mask or prosthetic facial covering, and restoration of the overall facial contour to preserve dignity during subsequent donor management. Restoration of the donor's facial integrity is both an ethical and legal obligation. A resin or 3D-printed mask can be fabricated from an alginate mold within approximately 30 min to recreate the donor's facial features. This procedure ensures a dignified presentation for funeral ceremonies and should be systematically performed as part of every facial allograft procurement.</li>
</ol>

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<li>Tong, J. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Normative+data+for+dimensions+of+the+frontal+and+infraorbital+nerves%5BTitle%5D)+AND+%22Eye+%28Lond%29%22%5BJournal%5D)">Normative data for dimensions of the frontal and infraorbital nerves.</a> Eye (Lond). 39 (7), 1390-1393 (2025).</li>
<li>Pomahac, B., Pribaz, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Facial+composite+tissue+allograft%5BTitle%5D)+AND+%22J+Craniofac+Surg%22%5BJournal%5D)">Facial composite tissue allograft.</a> J Craniofac Surg. 23 (1), 265-267 (2012).</li>
<li>Siemionow, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coronal-posterior+approach+for+face%2Fscalp+flap+harvesting+in+preparation+for+face+transplantation%5BTitle%5D)+AND+%22J+Reconstr+Microsurg%22%5BJournal%5D)">Coronal-posterior approach for face/scalp flap harvesting in preparation for face transplantation.</a> J Reconstr Microsurg. 22 (6), 399-405 (2006).</li>
<li>Smeets, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Face+transplantation%3A+On+the+verge+of+becoming+clinical+routine%5BTitle%5D)+AND+%22Biomed+Res+Int%22%5BJournal%5D)">Face transplantation: On the verge of becoming clinical routine.</a> Biomed Res Int. 2014, 907272(2014).</li>
<li>Siemionow, M., Gharb, B. B., Rampazzo, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pathways+of+sensory+recovery+after+face+transplantation%5BTitle%5D)+AND+%22Plast+Reconstr+Surg%22%5BJournal%5D)">Pathways of sensory recovery after face transplantation.</a> Plast Reconstr Surg. 127 (5), 1875-1889 (2011).</li>
<li>Sosin, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Total+face%2C+eyelids%2C+ears%2C+scalp%2C+and+skeletal+subunit+transplant%3A+A+reconstructive+solution+for+the+full+face+and+total+scalp+burn%5BTitle%5D)+AND+%22Plast+Reconstr+Surg%22%5BJournal%5D)">Total face, eyelids, ears, scalp, and skeletal subunit transplant: A reconstructive solution for the full face and total scalp burn.</a> Plast Reconstr Surg. 138 (1), 205-219 (2016).</li>
<li>Perraudin, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vascularized+composite+upper+limb+allograft+harvesting+for+proximal+arm+allotransplantation%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Vascularized composite upper limb allograft harvesting for proximal arm allotransplantation.</a> J Vis Exp. (220), e68170(2025).</li>
<li>Perraudin, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vascularized+composite+hand+allograft+procurement+and+preparation+for+distal+and+proximal+forearm+allotransplantation%3A+A+stepwise+approach%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Vascularized composite hand allograft procurement and preparation for distal and proximal forearm allotransplantation: A stepwise approach.</a> J Vis Exp. (219), e67767(2025).</li>
<li>Barbat, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Procurement+for+a+vascularized+and+reinnervated+abdominal+wall+allotransplantation%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Procurement for a vascularized and reinnervated abdominal wall allotransplantation.</a> J Vis Exp. (221), e68418(2025).</li>
<li>Pomahac, B., Bueno, E. M., Sisk, G. C., Pribaz, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Current+principles+of+facial+allotransplantation%3A+The+brigham+and+women%27s+hospital+experience%5BTitle%5D)+AND+%22Plast+Reconstr+Surg%22%5BJournal%5D)">Current principles of facial allotransplantation: The brigham and women's hospital experience.</a> Plast Reconstr Surg. 131 (5), 1069-1076 (2013).</li>
<li>Gurunian, R., Rampazzo, A., Papay, F., Gharb, B. b <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aGurunian%2C+R+%22Reconstructive+transplantation%22">Reconstructive transplantation</a>. , Springer. Nature, Switzerland. (2023).</li>
<li>Pomahac, B., Gobble, R. M., Schneeberger, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Facial+and+hand+allotransplantation%5BTitle%5D)+AND+%22Cold+Spring+Harb+Perspect+Med%22%5BJournal%5D)">Facial and hand allotransplantation.</a> Cold Spring Harb Perspect Med. 4 (3), a015651(2014).</li>
<li>Lupon, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+new+method+for+preparation+of+decellularized+human+scaffolds+for+facial+reconstruction%5BTitle%5D)+AND+%22Curr+Issues+Mol+Biol%22%5BJournal%5D)">A new method for preparation of decellularized human scaffolds for facial reconstruction.</a> Curr Issues Mol Biol. 47 (4), 275(2025).</li>
<li>Julienne, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((flap+harvest+training+on+a+new+ultrarealistic+simulation+model%3A+In-training+operator+feedback+about+a+pulsating+reperfused+and+reventilated+cadaver+simlife%C2%AE%5BTitle%5D)+AND+%22Ann+Chir+Plast+Esthet%22%5BJournal%5D)">flap harvest training on a new ultrarealistic simulation model: In-training operator feedback about a pulsating reperfused and reventilated cadaver simlife®.</a> Ann Chir Plast Esthet. 66 (2), 126-133 (2021).</li>
<li>Rodríguez-Lorenzo, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vascular+perfusion+of+the+facial+skin%3A+Implications+in+allotransplantation+of+facial+aesthetic+subunits%5BTitle%5D)+AND+%22Plast+Reconstr+Surg%22%5BJournal%5D)">Vascular perfusion of the facial skin: Implications in allotransplantation of facial aesthetic subunits.</a> Plast Reconstr Surg. 138 (5), 1073-1079 (2016).</li>
<li>Quilichini, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Restoration+of+the+donor+after+face+graft+procurement+for+allotransplantation%3A+Report+on+the+technique+and+outcomes+of+seven+cases%5BTitle%5D)+AND+%22Plast+Reconstr+Surg%22%5BJournal%5D)">Restoration of the donor after face graft procurement for allotransplantation: Report on the technique and outcomes of seven cases.</a> Plast Reconstr Surg. 129 (5), 1105-1111 (2012).</li>
<li>Baker, C. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pretreatments+of+ex+vivo+vascularized+composite+allografts%3A+A+scoping+review%5BTitle%5D)+AND+%22Ann+Plast+Surg%22%5BJournal%5D)">Pretreatments of ex vivo vascularized composite allografts: A scoping review.</a> Ann Plast Surg. , (2025).</li>
<li>Njessi, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Preservation+strategies+for+vascularized+composite+allotransplantation%3A+An+updated+systematic+review+of+a+rapidly+expanding+field%5BTitle%5D)+AND+%22bioRxiv%22%5BJournal%5D)">Preservation strategies for vascularized composite allotransplantation: An updated systematic review of a rapidly expanding field.</a> bioRxiv. , (2025).</li>
<li>Lupon, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+experimental+human+diep+flap+model+to+investigate+preservation+strategies+for+vascularized+composite+allografts+and+free+flaps%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">An experimental human diep flap model to investigate preservation strategies for vascularized composite allografts and free flaps.</a> J Vis Exp. (226), e69392(2025).</li>
<li>Lupon, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Engineering+vascularized+composite+allografts+using+natural+scaffolds%3A+A+systematic+review%5BTitle%5D)+AND+%22Tissue+Eng+Part+B+Rev%22%5BJournal%5D)">Engineering vascularized composite allografts using natural scaffolds: A systematic review.</a> Tissue Eng Part B Rev. , (2021).</li>
</ol>

The authors have no disclosure.

Face allotransplantation has become a milestone in reconstructive surgery, offering a unique solution for patients with otherwise unsalvageable facial defects4. Our harvested bipedicle graft included the skin, the eyelids, and the nose, together with the facial, supraorbital, infraorbital, and mental nerves. Both external carotid arteries should be isolated at their origins. In terms of neural structures, the main branches of the trigeminal nerve (supraorbital, infraorbital, and mental) s...

Nonfatal severe craniofacial defects profoundly affect patients&#39; social interactions and perceptions of their own self-image, often leading to significant psychological distress. Beyond appearance, intact facial anatomy is crucial for essential functions, including respiration, chewing, swallowing, speech, and non-verbal communication1,2.
Facial allotransplantation has emerged as a transformative reconstruction option for patients with severe facial deformities resulting from congenital anomalies, trauma, or oncologic resections. Despite advances in conventional reconstructive surgery, restoration of both function and aesthetics remains especially difficult for centrally located defects3,4.
Since the first partial face transplant in France in 20055, techniques have continued to evolve6,7, culminating most recently in 2023 with the landmark achievement of a combined whole-eye and face transplantation by Ceradini et al.8. Modern surgical planning and technological innovations have improved the reproducibility and predictability of outcomes, enabling better functional and aesthetic results4,9. However, lifelong immunosuppressive therapy introduces risks of infectious, metabolic and neoplastic complications, which raise substantial ethical and medical challengess10,11.
Procurement strategies have progressed from partial lower-face transplants12 to full face allografts8,13,14. Full-face procurement is indicated when extensive tissue loss involves multiple aesthetic and functional units, such as eyelids, nose, lips, cheeks, and when the restoration of vital functions (breathing, speech, mastication, swallowing) requires a three-dimensional reconstruction of the entire facial framework6,15.
Facial vascularization is mainly provided by the external carotid artery system, with the facial artery serving as the principal vessel and the superficial temporal artery as an important supplementary source16,17. Venous drainage occurs mainly via the facial vein into the internal jugular veins18. Preservation of neural structures is equally critical: motor function is restored through coaptation of the facial nerve while19 sensory innervation of the face depends on careful dissection of the trigeminal nerve, branches: V1, V2, and V320.
Here, we present a step-by-step protocol for harvesting a full-face vascularized composite allograft, including the forehead, eyelids, cheeks, nose, and lips from a cadaveric donor. The harvest incorporates only limited bony structures, restricted to the nasal framework, which is included primarily for pedagogical purposes to facilitate training reproducibility and minimize procedural complexity in cadaveric demonstrations.

<table><tbody><tr><td>11.5&amp;rdquo; Medium Premium Surgiclip II Auto Suture vessel clip applier</td><td>Covidien</td><td></td><td></td></tr><tr><td>2-0 silk suture&amp;nbsp;</td><td></td><td></td><td></td></tr><tr><td>Adson Forceps</td><td>MPM</td><td>106-2112A</td><td></td></tr><tr><td>Bioimpedance drill</td><td>Stryker</td><td>5400-50</td><td>High-speed drill with bioimpedance feedback for optic canal and skull base dissection</td></tr><tr><td>Bipolar Coagulation Forceps</td><td>Olsen</td><td>20-1320I</td><td></td></tr><tr><td>Custodiol HTK Solution for limb perfusion</td><td>Essential Pharmaceuticals Inc.</td><td></td><td>off-label use</td></tr><tr><td>Cysto/ Bladder Irrigation Set&amp;nbsp;</td><td>Baxter Healthcare Corp.</td><td></td><td></td></tr><tr><td>Disposable Scalpel #15</td><td>Sklar</td><td></td><td></td></tr><tr><td>DLP 3 mm vessel cannula blunt tip</td><td>Medtronic Inc</td><td></td><td></td></tr><tr><td>Forceps Dilators</td><td>WPI</td><td>15910</td><td></td></tr><tr><td>IV stopcock</td><td></td><td></td><td></td></tr><tr><td>Micro scissors</td><td>WPI</td><td>504492</td><td></td></tr><tr><td>Monopolar Diathermy</td><td>Valleylab</td><td></td><td></td></tr><tr><td>Oscillating saw</td><td>Stryker</td><td>5400-31</td><td>Standard oscillating saw for craniofacial osteotomies (also Synthes/DePuy)</td></tr><tr><td>Saline solution 0,9%</td><td>GenDepot</td><td>S0600-101</td><td></td></tr><tr><td>Strabismus scissors</td><td>Surtex</td><td>102-4109</td><td></td></tr><tr><td>Surgical marking pen</td><td>Cardinal health</td><td>212PR</td><td></td></tr><tr><td>Sutures Ethilon 4.0</td><td>Ethicon</td><td>1667G</td><td></td></tr><tr><td>Sutures Ethilon 4.0 and 9.0</td><td>Ethicon</td><td>1667G</td><td></td></tr><tr><td>Syringue 10 ml</td><td>Agilent</td><td>9301-6474</td><td></td></tr><tr><td>Three sterile procurement plastic bags, and three sterile zip ties</td><td></td><td></td><td></td></tr><tr><td>Tissue Forceps</td><td>MPM</td><td>106-0511</td><td></td></tr><tr><td>University of Wisconsin (UW) Solution for organ preservation</td><td>Bridge to Life</td><td></td><td>off-label use</td></tr><tr><td>Vessel loop</td><td>Deroyal</td><td>30-711</td><td></td></tr></tbody></table>

surgical approach to full soft tissue face allograft procurement for vascularized composite allotransplantation

Surgical Approach to Full Soft Tissue Face Allograft Procurement for Vascularized Composite Allotransplantation

Full facial vascularized composite allotransplantation (VCA) offers a reconstructive option for patients with severe facial disfigurement who cannot be treated with conventional methods. This article details a surgical protocol for donor procurement of a full facial allograft, focusing on soft tissue with limited bony harvest restricted to the nasal framework, and emphasizes key anatomical landmarks and technical considerations for safe and efficient harvest. The procedure involves dissection of the entire facial soft tissues while preserving the external carotid artery, internal jugular veins and subsequent thyro-linguofacial, and branches of the facial and maxillary nerves to maximize pedicle length for later neurorrhaphy. The protocol describes systematic exposure of the vascular pedicles at their origin in the neck, subperiosteal elevation of the scalp, identification and division of the supraorbital and infraorbital nerves, and preparation of the facial nerve at its root to achieve optimal length. Strategies to minimize ischemia time and ensure allograft integrity are highlighted. This article provides a comprehensive visual guide to the procurement phase of a full soft tissue face allograft and aims to standardize the technique for research application and surgical training in advanced vascularized composite allotransplantation.

The donor face dissected for this study was a male measuring 1.72 m in height with a malnourished body morphology. During graft dissection, vessel and nerve dimensions were measured bilaterally at the usual transection levels using a caliper, and mean values were calculated.
Mean vessel and nerve diameters from the donor were compared with averages reported in cadaveric and surgical literature. The harvested facial artery measured 1.5 mm at the mandibular border, closely matching cadaveric mea...

This protocol details the surgical technique used to harvest and prepare a full-face allograft for transplantation in patients with severe facial disfigurements, focusing primarily on soft tissue procurement with limited bony harvest restricted to the nasal framework.

Full facial vascularized composite allotransplantation (VCA) offers a reconstructive option for patients with severe facial disfigurement who cannot be ...

Our research focuses on standardizing full facial allograft procurement and identifying key anatomical landmarks to optimize safety, reproducibility, and training. Current challenges include minimizing ischemic time while achieving reproducible and safe dissections that preserve vascular and neural pedicle lengths. To begin the cervical dissection, use a number 15 scalpel blade to make a cervical incision extending from the superior border of the clavicle to the ear lobe.Using strabismus scissors, dissect the neck area to identify the sternocleidomastoid muscle. Perform a circumferential dissection off the external jugular vein. Ligate the external jugular vein and divide it using strabismus scissors.Next, with a Farabeuf retractor, retract the sternocleidomastoid muscle laterally to gain access to the vascular pedicles. Using strabismus scissors, dissect carefully to expose the internal jugular vein to reveal the thyrolinguofacial trunk and identify and preserve the facial vein. Dissect carefully to expose the common carotid artery to reveal the carotid bifurcation.Using strabismus scissors, dissect the external carotid artery cranially and identify the arterial branches. Ligate and divide the superior thyroid artery, ascending pharyngeal artery, and occipital artery. Continue the dissection to ligate and divide the lingual artery.Identify and preserve the facial branch of the external carotid artery. Now, identify and then transect the posterior belly of the digastric muscle and the stylohyoid muscle. Locate the hypoglossal nerve and section it proximally to obtain maximal length for potential nerve grafting.Next, ligate and divide the submandibular gland, duct, and vessels, excluding the submandibular gland from the allograft. For facial nerve dissection, use a number 15 scalpel blade to perform the pretracheal incision. Using strabismus scissors, dissect a preauricular skin flap in the sub-superficial musculoaponeurotic system plane.Transect the external auditory canal and expose the facial nerve at its origin. Then, using strabismus scissors, liberate all anterior soft tissues and block. Isolate the facial with a vessel loop and dissect the facial nerve with strabismus scissors to obtain maximal length.To begin the coronal approach, using a number 15 scalpel blade, make a coronal incision. Elevate the scalp flap in a subperiosteal plane from posterior to anterior, until the supraorbital nerve is encountered. Using strabismus scissors, ligate the supraorbital neurovascular contents close to the bony foramen.Divide the supraorbital contents, then ligate and divide the supratrochlear neurovascular contents close to the bony foramen. With the globe in situ, locate the levator palpebrae superioris muscle and place a marking suture using Ethilon on it. Next, proceed with periorbital dissection in the subperiosteal plane and divide the upper eyelid horizontally to preserve the orbicularis oculi within the graft.Perform circumferential transconjunctival incisions. Now, with an osteotome, elevate the lateral canthus with a small segment of the lateral orbital rim to ensure proper canthal support within the graft. Excise the lower eyelid while preserving the conjunctiva.Using strabismus scissors, dissect through the inferior fornix just inside the orbital rim. After identifying the infraorbital nerve, transect the infraorbital nerve near its foramen to ensure maximal nerve length for future neurorrhaphy. Employing a strabismus scissors, section the facial nerve at its exit from the stylomastoid foramen, and shave the masseter muscle to expose the mandibular surface.Raise the flap anteriorly on top of the masseter muscle. Identify the buccal fat pad at its anterior border, and continue the anterior dissection along the buccal fat pad while leaving it in situ. After harvesting the buccal mucosa in full, extend the dissection superiorly to the orbit and continue the dissection laterally to include the zygoma.To begin the midface osteotomies, perform a subperiosteal dissection lateral to the nose. Then, perform a low-to-low lateral nasal osteotomy, and then extend the osteotomy inferiorly to the hard palate with a curved osteotome. Next, extend the osteotomy laterally to include the zygomatic buttress.Divide the nasal septum longitudinally. Harvest the nose together with the skin and cartilage, including aller, triangular, and most of the septum, nasal bones, and nasal mucosa. For intraoral dissection, pick up a number 10 or number 15 scalpel blade to make a circumferential intraoral incision along the gingivobuccal mucosal junction.Then, incise the superior gingival mucosa following the maxillary dental arcade and the inferior gingival mucosa following the mandibular dental arcade. Now, incise the gingival mucosa along the mandibular line using the scalpel. After identifying the mental nerve near its mental foramen, divide it close to the foramen while preserving maximal length for potential neurorrhaphy.Now, using strabismus scissors, divide the mental nerve close to its foramen on the contralateral side. Mobilize the cervical soft tissue planes medially and direct the tissues toward the oral cavity. Dissect in the plane above the sternohyoid and superior omohyoid muscles with or without inclusion of the anterior jugular vein.Then, elevate the graft and block, and liberate the allograft from all remaining soft tissue attachments while preserving the bilateral vascular pedicles. Using absorbable monofilament, ligate the internal jugular veins as distally as possible. Ligate the carotid arteries as distally as possible.Finally, detach the entire face. The donor face dissected for this study was a male measuring 1.72 meters in height with a malnourished body morphology. The facial nerve was marked with a blue suture on each side of the graft.The internal view of the graft showed the orbital septum, superior gingival mucosa, and inferior gingival mucosa. The diameters of the harvested arteries and veins, including the facial artery, superficial temporal artery, facial vein, and internal jugular vein were generally consistent with or within the range of published literature values. The measured nerve diameters, including the facial nerve trunk and infraorbital nerve, were slightly smaller than published averages.This protocol fills a gap by providing a visual step-by-step reference, often more effective than extensive texts for complex surgical procedures. Our protocol provides a standardized and visually guided approach that improves reproducibility, anatomical understanding, and safety compared to narrative descriptions alone. Future research will focus on dynamic perfused models, like SimLife, to evaluate graft viability, perfusion, and surgical training realism.

surgical-approach-to-full-soft-tissue-face-allograft-procurement-for

Research

JoVE Journal

Medicine

Vascularized Composite Allotransplantations (VCA) have become a clinical reality. However, broad clinical application of VCA is limited by chronic multi-drug immunosuppression. The authors present a reliable and reproducible large animal model to translate novel immunomodulatory strategies that can minimize or potentially eliminate the need of immunosuppression in VCA.

A Modified Heterotopic Swine Hind Limb Transplant Model for Translational Vascularized Composite Allotransplantation (VCA) Research

Vascularized composite allograft offers life-altering benefits to transplant recipients, but the biological causes of graft rejection and vasculopathy remain poorly understood. The rodent surgical model presented here offers a reproducible, clinically relevant model of transplantation, allowing researchers to evaluate rejection events and potential therapeutic strategies to prevent their occurrence.

Modified Heterotopic Hindlimb Osteomyocutaneous Flap Model in the Rat for Translational Vascularized Composite Allotransplantation Research

The present protocol describes the porcine fascio-cutaneous flap model and its potential use in vascularized composite tissue research.

A Reliable Porcine Fascio-Cutaneous Flap Model for Vascularized Composite Allografts Bioengineering Studies

The protocol describes the surgical procurement and subsequent decellularization of vascularized porcine flaps by the perfusion of sodium dodecyl sulfate detergent through the flap vasculature in a customized perfusion bioreactor.

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

We describe the surgical technique and decellularization process for composite rat hindlimbs. Decellularization is conducted using low-concentration sodium dodecyl sulfate through an ex vivo machine perfusion system.

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

Here, we present a protocol for procuring and preparing vascularized composite hand allografts during distal or proximal forearm transplantation.

Vascularized Composite Hand Allograft Procurement and Preparation for Distal and Proximal Forearm Allotransplantation: A Stepwise Approach

Here, the protocol explains step-by-step the technique of harvesting an upper arm allograft at the trans humeral or gleno-humeral level during an arm allotransplantation.

Vascularized Composite Upper Limb Allograft Harvesting for Proximal Arm Allotransplantation

This protocol describes a step-by-step procedure for harvesting a vascularized and reinnervated abdominal wall allograft, detailing its anatomic landmarks, technical requirements, and future potential clinical applications.

Procurement for a Vascularized and Reinnervated Abdominal Wall Allotransplantation

This protocol describes the surgical technique for harvesting a human perforator flap model based on the deep inferior epigastric artery pedicle, intended for experimental research.

An Experimental Human DIEP Flap Model to Investigate Preservation Strategies for Vascularized Composite Allografts and Free Flaps

We present a reproducible mouse model of heterotopic hindlimb-to-neck vascularized composite allotransplantation. This supermicrosurgical protocol, involving 0.3-0.5 mm vessel anastomoses, integrates nylon filament guidance and venous cuffing. It provides a reliable platform for translational studies on tolerance, ischemia-reperfusion injury, and immunological mechanisms in vascularized composite allotransplantation.

Heterotopic Hindlimb-to-Neck Transplantation in the Mouse: A Supermicrosurgical Model of Vascularized Composite Allotransplantation

Learn the surgical protocol for full soft tissue face allograft procurement using cervical and coronal dissection techniques. This standardized approach preserves vascular and neural structures for vascularized composite allotransplantation.

Surgical Approach to Full Soft Tissue Face Allograft Procurement for Vascularized Composite Allotransplantation

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