/

/

A Reliable Porcine Fascio-Cutaneous Flap Model for Vascularized Composite Allografts Bioengineering Studies

JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering

A Reliable Porcine Fascio-Cutaneous Flap Model for Vascularized Composite Allografts Bioengineering Studies

A Reliable Porcine Fascio-Cutaneous Flap Model for Vascularized Composite Allografts Bioengineering Studies

DOI:

10.3791/63557-v

•

05:34 min

•

March 31, 2022

•

Victor Pozzo^2,4, Golda Romano^2,4, Marion Goutard^2,4, Elise Lupon^2,4, Pierre Tawa^2,4, Aylin Acun^4,5, Alec R. Andrews, Corentin B. Taveau^2,4,6, Basak E. Uygun^2,3,4, Mark A. Randolph^2,4, Curtis L. Cetrulo^2,4, Alexandre G. Lellouch^2,4,6

¹Division of Plastic and Reconstructive Surgery,Massachusetts General Hospital, Harvard Medical School, ²Vascularized Composite Allotransplantation Laboratory, Center for Transplantation Sciences,Massachusetts General Hospital, Harvard Medical School, ³Center for Engineering in Medicine and Surgery,Massachusetts General Hospital, Harvard Medical School, ⁴Shriners Hospital for Children, ⁵Department of Biomedical Engineering,Widener University, ⁶Service de Chirurgie Plastique, Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris (APHP),Université Paris Descartes

Chapters

00:04Introduction
00:39Bilateral Saphenous Fascio‐Cutaneous Flaps (FCF) Procurement
04:04Results: A Fascio‐Cutaneous Flap (FCF) Model for Allografts Bioengineering Studies
04:57Conclusion

Summary

Automatic Translation

The present protocol describes the porcine fascio-cutaneous flap model and its potential use in vascularized composite tissue research.

Tags

Keywords: Porcine Fasciocutaneous Flap Vascularized Composite Allograft Saphenous Artery Femoral Vessels Surgical Technique Large Animal Model Immunology

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions

Engineered Vascularized Muscle Flap

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering

Quantification of Strain in a Porcine Model of Skin Expansion Using Multi-View Stereo and Isogeometric Kinematics

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

A Reliable and Reproducible Critical-Sized Segmental Femoral Defect Model in Rats Stabilized with a Custom External Fixator

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

Generation of Self-assembled Vascularized Human Skin Equivalents

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Read Article