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In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging
 

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging

Article DOI: 10.3791/827-v 11:41 min August 3rd, 2008
August 3rd, 2008

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Summary

In this video, we are showing how to label human embryonic stem cells (hESC) with manganese chloride (MnCl2) which can enter cells via voltage-gated calcium channels when the cells are biologically active. Additionally, we show the use of MnCl2 as cellular MRI contrast agent to determine the in vitro viability of hESC.

Tags

In Vitro Labeling Human Embryonic Stem Cells Magnetic Resonance Imaging MRI Iron-oxide Nanoparticles Transplanted Stem Cells Manganese Chloride Ca2+ Channels T1 Shortening Effect Cell Viability Myocardium In Vitro MRI Imaging
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