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4D Microscopy of Yeast

4D Microscopy of Yeast

Article DOI: 10.3791/58618-v
April 28th, 2019


Summary April 28th, 2019

This protocol describes the analysis of fluorescently labeled intracellular compartments in budding yeast using multi-color 4D (time-lapse 3D) confocal microscopy. The imaging parameters are chosen to capture adequate signals while limiting photodamage. Custom ImageJ plugins allow labeled structures to be tracked and quantitatively analyzed.


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