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A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
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A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Article DOI: 10.3791/56091-v • 11:27 min • November 2nd, 2018
November 2nd, 2018 •
1,2, 1, 1, 1
1Department of Chemistry & Biochemistry, University of California, San Diego, 2Department of Biophysics, Bose Institute

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Summary
Automatic Translation

IκB Kinase 1/α (IKK1/α CHUK) is a Ser/Thr protein kinase that is involved in a myriad of cellular activities primarily through activation of NF-κB transcription factors. Here, we describe the main steps necessary for the production and crystal structure determination of this protein.

Tags

Production Crystallization Structure Determination Human IKK1-alpha Signaling Function Rational Drug Design Immune Signaling Crystals Protein Expression Insect Cells Narrow Window Of Conditions Molecular Replacement Procedure Deflection Property Asymmetric Unit Search Models Sf9 Cells Sf903 Insect Cell Medium Incubate
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