Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Characterization of Glycoproteins with the…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae
Journal (Biochemistry)
Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
Journal (Biochemistry)
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
 

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

Article DOI: 10.3791/57750-v • 08:58 min • July 5th, 2018
July 5th, 2018 •
*1, *1,2, 1, 1, 1,2,3
1Program in Molecular Medicine, The Hospital for Sick Children Research Institute, 2Department of Biochemistry, University of Toronto, 3Department of Immunology, University of Toronto
* These authors contributed equally

Chapters

Summary
Automatic Translation

We present approaches for the biophysical and structural characterization of glycoproteins with the immunoglobulin fold by biolayer interferometry, isothermal titration calorimetry, and X-ray crystallography.

Tags

Glycoproteins Immunoglobulin Fold X-ray Crystallography Biophysical Techniques Function Of Protein N-linked Glycans Antibiotics Targeting Glycoprotein Structural Characterization Biophysical Characterization Hong Cui Research Project Coordinator Cell Transfection Centrifugation Filtration Ni-NTA Column Chromatography System Wash Buffer Elution Buffer Gradient Centrifugal Filtration Device
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter