Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Subtype-specific Optical Action Potential…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes
Automated Detection and Analysis of Exocytosis
Journal (Biology)
Automated Detection and Analysis of Exocytosis
Staining Proteins in Gels
Journal (Biology)
Staining Proteins in Gels
 

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

Article DOI: 10.3791/58134-v • 06:59 min • September 27th, 2018
September 27th, 2018 •
*1,2, *1, 1, 3, 1,2, 1,2
1Medical Department I, University Hospital Klinikum rechts der Isar, Technical University of Munich, 2German Centre for Cardiovascular Research (DZHK), Munich Heart Alliance, 3Beth Israel Deaconess Medical Center, Harvard Medical School
* These authors contributed equally

Chapters

Summary
Automatic Translation

Here we present a method to optically image action potentials, specifically in ventricular-like induced pluripotent stem cell-derived cardiomyocytes. The method is based on the promoter-driven expression of a voltage-sensitive fluorescent protein.

Tags

Optical Action Potential Recordings Induced Pluripotent Stem Cells Ventricular Cardiomyocytes Cardiac Electrophysiology Subtype-specific Action Potentials Genetically Encoded Voltage Sensors Lentiviral Transduction Infection Efficiency Cardiomyocyte Maintenance Medium IPS Cell-derived Cardiomyocytes Culture Tyrode's Solution Epifluorescence Microscope
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter