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Immunology and Infection

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Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

Article DOI: 10.3791/59314-v • 12:15 min • April 9th, 2019
April 9th, 2019 •
*1,2, *3,4, 3,5, 3, 1,6, 3, 3
1Department of Macromolecular Structures, Centro Nacional de Biotecnología, Campus Univ. Autónoma de Madrid, 2Campus Urb. Montepríncipe s/n, Univ. San Pablo CEU, 3Department of Immunology and Oncology, Centro Nacional de Biotecnología, Campus Univ. Autónoma de Madrid, 4Department of Cell Signaling, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CSIC), 5Meyer Cancer Center, 6Department of Anatomy and Cell Biology, McGill Univ.
* These authors contributed equally

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Summary
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Here, we present a protocol for single particle tracking image analysis that allows quantitative evaluation of diffusion coefficients, types of motion and cluster sizes of single particles detected by fluorescence microscopy.

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Image Processing Protocol Analysis Diffusion Cluster Size Membrane Receptors Fluorescence Microscopy Single-molecule Tracking Lateral Diffusion Parameters Spot Size Quantification Cell Membrane Software Tools ImageJ MATLAB UTrack Proteins Light Microscopy Basic Research Clinical Setting Therapeutic Targets Cellular Membranes Full Cells Bacteria Nanoparticles Fluid User-friendly
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