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JoVE Journal
Biology

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Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
 

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Article DOI: 10.3791/52208
February 8th, 2015

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Summary February 8th, 2015

Studies of biomolecules in vivo are crucial for understanding molecular function in a biological context. Here we describe a novel method allowing the internalization of fluorescent biomolecules, such as DNA or proteins, into living microorganisms. Analysis of in vivo data recorded by fluorescence microscopy is also presented and discussed.

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