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February 08, 2015
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Studies of biomolecules in vivo are crucial for understanding molecular function in a biological context. Here we describe a novel method allowing the internalization of fluorescent biomolecules, such as DNA or proteins, into living microorganisms. Analysis of in vivo data recorded by fluorescence microscopy is also presented and discussed.
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Cite this Article
Aigrain, L., Sustarsic, M., Crawford, R., Plochowietz, A., Kapanidis, A. N. Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation. J. Vis. Exp. (96), e52208, doi:10.3791/52208 (2015).
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