A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:40Plasmid Construction and Bacterial Cell Culture
- 02:24Cell Lysis and Protein Purification
- 05:16Spinning Dope Preparation, Syringe Preparation, and Apparatus Set-up
- 06:57Post-spin Draw and Sample Collection
- 09:28Tensile Testing
- 10:57Analysis of Synthesized Spider Silk
- 12:24Conclusion
Trods de fremragende mekaniske og biokemiske egenskaber af spindemider silke, kan dette materiale ikke kan høstes i store mængder ved hjælp af konventionelle midler. Her beskriver vi en effektiv strategi til at spinde kunstige edderkoppespind fibre, hvilket er en vigtig proces for efterforskerne at studere edderkoppespind produktion og deres anvendelse som næste generation biomaterialer.
Tags
Synthetic Spider SilkLaboratory ScaleBiomaterialsHigh PerformanceStructural MaterialsProcessing MethodologiesEnvironmentally FriendlySustainableBiomimicryEngineering MaterialsSynthetic Silk ProductionSpinning ProcessArtificial Spider FibersBody ArmorSurgical SuturesRopes And CablesTiresMusical InstrumentsCompositesAviation And Aerospace TechnologyWet-spinning ProtocolRecombinant Spider Silk ProteinsFiber Extrusion
