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Chapters
- 00:05Title
- 01:40Plasmid Construction and Bacterial Cell Culture
- 02:24Cell Lysis and Protein Purification
- 05:16Spinning Dope Preparation, Syringe Preparation, and Apparatus Set-up
- 06:57Post-spin Draw and Sample Collection
- 09:28Tensile Testing
- 10:57Analysis of Synthesized Spider Silk
- 12:24Conclusion
Despite the outstanding mechanical and biochemical properties of spider silks, this material cannot be harvested in large quantities by conventional means. Here we describe an efficient strategy to spin artificial spider silk fibers, which is an important process for investigators studying spider silk production and their use as next-generation biomaterials.
Tags
Synthetic Spider SilkLaboratory ScaleBiomaterialsHigh PerformanceStructural MaterialsProcessing MethodologiesEnvironmentally FriendlySustainableBiomimicryEngineering MaterialsSynthetic Silk ProductionSpinning ProcessArtificial Spider FibersBody ArmorSurgical SuturesRopes And CablesTiresMusical InstrumentsCompositesAviation And Aerospace TechnologyWet-spinning ProtocolRecombinant Spider Silk ProteinsFiber Extrusion
