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Chapters
- 00:00Title
- 00:45Introduction
- 01:14Animal Care and Genetic Crosses
- 02:48Electrophysiology Rig Setup
- 04:03Larval Dissection
- 06:37Blue Light Stimulation and Muscle Recording
- 07:54Representative Results: Light Induced E.J.P.s
- 08:28Conclusion
This procedure uses a blue light-activated algal channel and cell-specific genetic expression tools to evoke synaptic potentials with light pulses at the neuromuscular junction (NMJ) in Drosophila larvae. This technique is an inexpensive and easy-to-use alternative to suction electrode stimulation for synaptic physiology studies in research and teaching laboratories.
