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Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at Drosophila Neuromuscular Junctions
JoVE Journal
Biology
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JoVE Journal Biology
Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at Drosophila Neuromuscular Junctions
DOI:

09:12 min

March 16, 2009

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Chapters

  • 00:00Title
  • 00:45Introduction
  • 01:14Animal Care and Genetic Crosses
  • 02:48Electrophysiology Rig Setup
  • 04:03Larval Dissection
  • 06:37Blue Light Stimulation and Muscle Recording
  • 07:54Representative Results: Light Induced E.J.P.s
  • 08:28Conclusion

Summary

Automatic Translation

This procedure uses a blue light-activated algal channel and cell-specific genetic expression tools to evoke synaptic potentials with light pulses at the neuromuscular junction (NMJ) in Drosophila larvae. This technique is an inexpensive and easy-to-use alternative to suction electrode stimulation for synaptic physiology studies in research and teaching laboratories.

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