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Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS
DOI:

11:02 min

May 12, 2011

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Chapters

  • 00:05Title
  • 01:13Introduction
  • 01:44Syringe Preparation for Intraocular Injections
  • 04:48Intraocular Injection Procedure: Targeting the Retina Globally
  • 06:50Selectively Targeting Retinal Ganglion Cells from the Optic Nerve Stump
  • 09:28RGC Targeting by Injection of the Transected Optic Nerve Stump
  • 10:31Conclusion

Summary

Automatic Translation

Optic Nerve transection is a widely used model of adult CNS injury. This model is ideal for performing a number of experimental manipulations that target the retina globally or directly target the injured neuronal population of retinal ganglion cells.

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