JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos
In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos
DOI:
10.3791/236-v
•
19:04 min
•
July 19, 2007
•
William Walantus
,
Laura Elias
,
Arnold Kriegstein
1
Institute for Regeneration Medicine
,
University of California, San Francisco – UCSF
Chapters
00:00
Title
00:17
Introduction
01:06
Tools and Reagents
02:17
Preparation of Microinjection Pipets
06:23
Prepping Rat for Surgery
10:02
Removing Uterus and Exposing Embryos
12:12
In utero Injection and Electroporation
14:11
Placing Embryos Back Inside Dam
17:23
Conclusion
18:48
End Credits
Tags
In-utero Intraventricular Injection
Electroporation
E16 Rat Embryos
Neocortex Manipulation
Progenitor Cells
Lateral Ventricle
Corticogenesis Study
Gene Over-expression
Gene Knockdown
Cellular Proliferation
Migration
Differentiation
Knockout Strategies
Temporal Window
Surgery Preparation
Laporatomy
DNA Injection
Progenitor Cells Lining The Lateral Wall
Post-surgery Care
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
Injection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes
In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
Electroporation of Mycobacteria
In utero
and
ex vivo
Electroporation for Gene Expression in Mouse Retinal Ganglion Cells
Preparation of embryos for Electron Microscopy of the
Drosophila
embryonic heart tube
Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development
RNAi Interference by dsRNA Injection into
Drosophila
Embryos
Imaging Cell Shape Change in Living
Drosophila
Embryos
Isolation and Primary Culture of Rat Hepatic Cells
Read Article