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Journal
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Biology
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In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
JoVE Journal
Biology
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JoVE Journal
Biology
In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
DOI:
10.3791/239-v
•
26:17 min
•
July 19, 2007
•
William Walantus
,
David Castaneda
,
Laura Elias
,
Arnold Kriegstein
1
Institute for Regeneration Medicine
,
University of California, San Francisco – UCSF
Chapters
00:00
Title
00:17
Introduction
01:17
Tools and Reagents
02:33
Anesthesia Procedure
03:13
Preparation of Microinjection Pipets
07:18
Prepping Rat for Surgery
09:59
Removing Uterus and Exposing Embryos
12:11
In utero Injection and Electroporation
16:49
Placing Embryos Back Inside Dam
23:46
Conclusion
26:01
End Credits
Tags
In-utero
Intraventricular Injection
Electroporation
E15 Mouse Embryos
Neocortex Manipulation
Progenitors
Lateral Ventricle
Corticogenesis
Gene Over-expression
Gene Knockdown
Cellular Proliferation
Migration
Differentiation
Knockout Strategies
Temporal Window
Video Protocol
Surgery Preparation
Uterine Horns Exposure
DNA Injection
Progenitor Electroporation
Animal Care Post-surgery
Article
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