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Journal
/
Biology
/
Modified ES / OP9 Co-Culture Protocol Provides Enhanced Characterization of Hematopoietic Progeny
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Modified ES / OP9 Co-Culture Protocol Provides Enhanced Characterization of Hematopoietic Progeny
Modified ES / OP9 Co-Culture Protocol Provides Enhanced Characterization of Hematopoietic Progeny
DOI:
10.3791/2559-v
•
08:36 min
•
June 07, 2011
•
Maureen R. Lynch
,
Judith C. Gasson
,
Helicia Paz
1
Department of Medicine, Hematology-Oncology
,
University of California, Los Angeles
,
2
Department of Biological Chemistry
,
University of California, Los Angeles
Chapters
00:05
Title
01:31
Preparing mStrawberry OP9 Cells
02:32
Setting up ES/mStrawberry OP9 Co-culture (Day 0)
03:55
Maintaining ES/mStrawberry OP9 Co-culture
06:16
Evaluating ES-derived Progeny from the Co-culture System
Summary
Automatic Translation
English (Original)
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Automatic Translation
mStrawberry OP9 cells allow for complete evaluation of all ES-derived progeny from co-culture.
Tags
ES/OP9 Co-culture Protocol
Hematopoietic Progeny
In Vitro Differentiation
Early Hematopoietic Development
Flk-1+ Hemangioblasts
Terminally Differentiated Lineages
OP9 Stromal Cells
M-CSF
Embryonic Lethalities
Macrophage Overproduction
Replating Steps
Evaluation Of ES-derived Progeny
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