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Microfabricated Post-Array-Detectors (mPADs): an Approach to Isolate Mechanical Forces
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JoVE Journal Biology
Microfabricated Post-Array-Detectors (mPADs): an Approach to Isolate Mechanical Forces

Microfabricated Post-Array-Detectors (mPADs): an Approach to Isolate Mechanical Forces

DOI:
10.3791/311-v

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01:34 min

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October 01, 2007

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, , , ,
1Department of Bioengineering,University of Pennsylvania , 2University of Washington

Chapters

  • 00:06Introduction
  • 02:53Fabrication
  • 14:01Replica Molding
  • 24:36Functionalizing mPADs
  • 43:50Live observation
  • 51:04Fixed observation
  • 54:52Traction forces analysis
  • 59:54Conclusion

Summary

Automatic Translation

Automatic Translation

In this video, we demonstrate how to fabricate and utilize microfabricated post array detectors (mPADs) to assess modulations of cellular contractility.

Tags

Microfabricated Post-array-detectorsMPADsMeasure Cellular Traction ForcesMyosin-actin InteractionsPhysiologyDevelopmentHealing ProcessesWound ClosureLeukocyte MigrationCancer MetastasisVascular GrowthIn Vitro Cell StudyGlass DishPolystyrene DishSubstrate RigidityCell Traction Force MeasurementFlexible CantileversPillar Dimensions

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