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Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
JoVE Journal
Medicine
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JoVE Journal Medicine
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
DOI:

15:43 min

October 25, 2011

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Chapters

  • 00:05Title
  • 02:46Isolation of Primary Cultured Human Normal Mammary Fibroblasts
  • 04:43Generation of GFP-labeled, Puromycin-Resistant and Immortalized Human Normal Mammary Fibroblasts
  • 07:35Co-injection of Human Mammary Fibroblasts with Breast Carcinoma Cells into an Immunodeficient Mouse
  • 08:58Extraction of CAFs or their Corresponding Control Fibroblasts from the Tumor or Fibroblast Xenografts, Respectively
  • 13:31Representative Images of Experimentally Generated CAFs
  • 15:18Conclusion

Summary

Automatic Translation

Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.

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