Journal
/
/
Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach
DOI:

08:29 min

March 27, 2012

,

Chapters

  • 00:05Title
  • 01:20Culturing Human Embryonic Stem Cells
  • 02:05Definitive Endoderm and Pancreatic Progenitor Induction
  • 03:01Pancreatic Maturation
  • 03:57qRT-PCR Analysis
  • 05:06Immunocytochemistry
  • 06:21Analysis of Differentiation of Human Embryonic Stem Cells into Insulin-producing Cells
  • 08:07Conclusion

Summary

Automatic Translation

The current study describes a directed differentiation approach in inducing pancreatic differentiation of human embryonic stem cells. Of great significance is the finding that endothelial cell co-culture mediates maturation of human embryonic stem cell derived pancreatic progenitors into insulin expressing cells.

Related Videos

Read Article