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Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer
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Bioengineering
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JoVE Journal Bioengineering
Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer

Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer

DOI:
10.3791/4398-v

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10:59 min

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November 02, 2012

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,
1Human Immune Monitoring Center, Institute for Immunity, Transplantation, and Infection,Stanford University

Chapters

  • 00:05Title
  • 00:50Start-up of the Mass Cytometer
  • 01:59Daily Calibration of the Mass Cytometer
  • 05:21Running Samples
  • 06:54Cleaning and Shutting Down the Machine After Use
  • 07:58Results: The Effect of Proper Tuning on the Desired Signal and the Difference in Oxide Formation Between Various Lanthanide Metals
  • 10:39Conclusion

Summary

Automatic Translation

Automatic Translation

The steps necessary for daily tuning and optimization of the performance of a CyTOF mass cytometer are described. Comments on optimal sample preparation and flow rate are discussed

Tags

Mass CytometryCyTOF Mass CytometerFlow CytometryFluorescently-labeled AntibodiesSpectral OverlapChelating PolymersMetal IsotopesCompensation MatricesLanthanide MetalsAtomic MassImmunological ProfileChelating ReagentsDestructive Technique

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