/
/
Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:54Flow Chamber Setup and Cell Seeding
- 05:15AFM Cantilever Preparation and Cell Indentation
- 07:45Calculation of Glycocalyx Layer Thickness
- 08:38Results: AFM Measurement of Glycocalyx Layer Thickness
- 09:32Conclusion
The mechanical characteristics of endothelial glycocalyx were measured by indentation using micron sized spheres on AFM cantilevers. Endothelial cells were cultured in a custom chamber under physiological flow conditions to induce glycocalyx expression. Data were analyzed using a thin film model to determine the glycocalyx thickness and modulus.
