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Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
DOI:

08:46 min

January 15, 2014

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Chapters

  • 00:05Title
  • 01:14Growing Single Ice Crystals
  • 03:18Determining Singularity and Orientation o the Ice Crystal
  • 04:37Absorption of Fluorescently Labeled Antifreeze Proteins to a Single Ice Crystal
  • 07:20Results: Fluorescence-based Ice Plane Affinity Analysis Images
  • 08:18Conclusion

Summary

Automatic Translation

Antifreeze proteins (AFPs) bind to specific planes of ice to prevent or slow ice growth. Fluorescence-based ice plane affinity (FIPA) analysis is a modification of the original ice-etching method for determination of AFP-bound ice planes. AFPs are fluorescently labeled, incorporated into macroscopic single ice crystals, and visualized under UV light.

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