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Chapters
- 00:05Title
- 01:57Three Dimensional Culture Preparation
- 03:34Quantification of the Malignant vs. Benign Breast Phenotype
- 04:31Immunofluorescence Staining of Acinar Structures Grown on Matrigel
- 06:36Results: Comparison of Benign vs. Malignant Phenotypes
- 08:05Conclusion
Three dimensional culture of mammary epithelial cells on a reconstituted basement membrane is a useful method to recapitulate the in vivo architecture of the benign breast, and to differentiate the malignant phenotype from the benign breast phenotype. Importantly, this system can be applied to study invasive carcinomas in other tissues.
