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Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of Individual Cortical Neurons
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of Individual Cortical Neurons
DOI:

10:36 min

February 25, 2014

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Chapters

  • 00:05Title
  • 01:45Animal Preparation
  • 03:38Juxtasomal Recordings and Biocytin Labeling
  • 08:22Results: Imaging of Labeled Neurons
  • 09:58Conclusion

Summary

Automatic Translation

To understand the structure of neuronal networks, functional and morphological characterization of individual neurons is a necessity. Here, we demonstrate juxtasomal biocytin labeling, which allows electrophysiological recordings in the extracellular configuration, yet maintaining the ability to intracellularly label the neuron for post hoc reconstruction of dendritic and axonal architecture.

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