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Spinal Cord Transection in the Larval Zebrafish
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Spinal Cord Transection in the Larval Zebrafish
DOI:

06:57 min

May 21, 2014

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Chapters

  • 00:05Title
  • 01:11Preparation of Surgery Plates and Micropipettes
  • 02:26Preparation of Zebrafish Larvae
  • 03:47Surgery
  • 05:03Recovery
  • 05:55Results: Verifying a Complete Transection
  • 06:38Conclusion

Summary

Automatic Translation

After spinal transection, adult zebrafish have functional recovery by six weeks post-injury. To take advantage of larval transparency and faster recovery, we present a method for transecting the larval spinal cord. After transection, we observe sensory recovery beginning at 2 days post-injury, and C-bend movement by 3 days post-injury.

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