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Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
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Genetics
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JoVE Journal Genetics
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

DOI:
10.3791/54976-v

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09:57 min

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December 28, 2016

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, ,
1Entomology Department,University of Maryland, 2Program in Molecular and Cell Biology,University of Maryland

Chapters

  • 00:05Title
  • 01:00D. maculatus Rearing
  • 01:37D. maculatus Embryo Collection
  • 02:58Embryonic RNAi (eRNAi)
  • 05:14Parental RNAi (pRNAi)
  • 07:04Results: Roles of Dmac-prd in Body Segment Formation Were Demonstrated Using pRNAi
  • 08:38Conclusion

Summary

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Here, we present protocols for rearing an intermediate-germ beetle, Dermestes maculatus (D. maculatus) in the lab. We also share protocols for embryonic and parental RNAi and methods for analyzing embryonic phenotypes to study gene function in this species.

Tags

Dermestes MaculatusHide BeetleRearingDouble-stranded RNAGene KnockdownInsect Model SystemRNA InterferenceDevelopmental BiologyEvolutionary BiologyInsect Pest Control

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