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Immunology and Infection

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JoVE Journal Immunology and Infection
An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
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An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

Article DOI: 10.3791/55559-v • 12:17 min • May 8th, 2017
May 8th, 2017 •
1, 1, 2, 2, 2, 1
1Public Health Research Institute Centre, New Jersey Medical School, Rutgers, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, NIAID, NIH

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Summary
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Here we describe a rapid equilibrium dialysis (RED) method to measure drug binding to caseum from pulmonary tuberculosis lesions and cavities. The protocol is also used with a foamy macrophage-derived matrix that is an effective surrogate to caseum.

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In Vitro Caseum Binding Assay Drug Penetration Tuberculosis Lesions Antimicrobials Necrotic Lesions Animal Models Imaging Techniques Tuberculosis Drug Discovery Lesions Abscesses Surrogate Matrix Cell Culture Procedure THP-1 Monocytes RPMI 1640 Medium L-glutamine Fetal Bovine Serum Centrifuge Supernatant Trypan Blue
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