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Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
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JoVE Journal Engineering
Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

DOI:
10.3791/55840-v

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07:19 min

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June 28, 2017

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, , , , , , , ,
1Department of Electrical and Electronic Engineering,The University of Hong Kong, 2Department of Mechanical Engineering,The University of Hong Kong

Chapters

  • 00:05Title
  • 00:51ATOM System Setup
  • 03:20Data Acquisition and Analysis
  • 04:56Results: Single-cell Imaging of Mammalian Cells and Phytoplanktons with ATOM
  • 06:00Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the implementation of an asymmetric-detection time-stretch optical microscopy system for single-cell imaging in ultrafast microfluidic flow and its applications in imaging flow cytometry.

Tags

MicrofluidicImaging Flow CytometryAsymmetric-detectionTime-stretch Optical MicroscopyATOMLabel-freeHigh ContrastSingle-cell ImagingUltra-fast Microfluidic FlowFiber CollimatorBroadband Femtosecond/picosecond Near-IR Pulsed LaserSingle Mode Dispersive Optical FiberOptical AmplifierTransmission Diffraction GratingTelescopic Relay Lens4f Imaging SystemObjective LensMicrofluidic ChipBeam SplitterFiber CouplerPhotodetectorOscilloscope

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