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Chapters
- 00:05Title
- 01:24Plant Seed Surface Sterilization
- 03:03Seed Germination and Semi-solid Cultivation of Plants
- 04:51Hydroponic Co-cultivation System
- 07:39Fluorescence Microscopy
- 08:24Secretome Profiling
- 09:10Results: Co-cltivation System Mimics Natural Environment
- 10:28Conclusion
The described hydroponic cocultivation system supports intact plants with metal mesh screens and cocultivates them with bacteria. Plant tissue, bacteria, and secreted molecules can then be separately harvested for downstream analyses, simultaneously allowing for the molecular responses of both plant hosts and interacting microbes or microbiomes to be investigated.
Tags
Hydroponic Co-cultivationPlant-microbe InteractionsMolecular SignalingAgrobacterium TumefaciensArabidopsis ThalianaSeed SterilizationMurashige And Skoog (MS) MediumPhyto AgarPlant-microbe CommunicationReciprocal SignalingAbiotic FactorsNatural Environment SimulationSingle/multiple Microbial SpeciesCell Signaling Homeostasis
