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A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
JoVE Journal
Biology
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JoVE Journal Biology
A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
DOI:

11:16 min

July 22, 2017

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Chapters

  • 00:05Title
  • 01:24Plant Seed Surface Sterilization
  • 03:03Seed Germination and Semi-solid Cultivation of Plants
  • 04:51Hydroponic Co-cultivation System
  • 07:39Fluorescence Microscopy
  • 08:24Secretome Profiling
  • 09:10Results: Co-cltivation System Mimics Natural Environment
  • 10:28Conclusion

Summary

Automatic Translation

The described hydroponic cocultivation system supports intact plants with metal mesh screens and cocultivates them with bacteria. Plant tissue, bacteria, and secreted molecules can then be separately harvested for downstream analyses, simultaneously allowing for the molecular responses of both plant hosts and interacting microbes or microbiomes to be investigated.

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