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Preparation of Dissociated Mouse Cortical Neuron Cultures
JoVE Journal
Biology
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JoVE Journal Biology
Preparation of Dissociated Mouse Cortical Neuron Cultures
DOI:

18:02 min

December 19, 2007

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Chapters

  • 00:00Title
  • 00:15Introduction
  • 01:06Tools and Reagents
  • 03:13Mounting Brain onto Vibraslicer
  • 04:26Removing and Sectioning Brain
  • 08:12Isolating and Processing Cortex
  • 11:29Washing Cortical Pieces
  • 13:14Trituration of Cortical Pieces
  • 16:35Visualizing Cortical Neurons
  • 17:12Conclusion

Summary

Automatic Translation

This video shows a procedure for generating neuronal cultures from late embryo and early postnatal mouse cortex. These cultures can be used for immunocytochemistry, biochemistry, electrophysiology, calcium and sodium imaging and provide a platform to study the neuronal development of transgenic animals that carry a postnatal lethal gene mutation.

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