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JoVE Journal
Immunology and Infection

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Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death
 

Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death

Article DOI: 10.3791/56535-v 08:26 min October 31st, 2017
October 31st, 2017

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Summary

We illustrate a straight-forward method to derive murine primary macrophages from bone marrow cells and a simple method to prepare BSA-fatty acid conjugates. Then we demonstrate that saturated fatty acids can induce macrophage cell death, and such cell death is positively associated with cellular accumulation of ceramide levels.

Tags

Saturated Fatty Acids Ceramides Macrophage Cell Death Metabolic Inflammation BSA Fatty Acid Conjugates Tibia And Femur Bones Red Blood Cell Lysis Buffer PBS
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