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JoVE Journal Genetics
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Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Article DOI: 10.3791/56728-v • 10:29 min • March 30th, 2018
March 30th, 2018 •
1, 1, 1, 1, 1
1Department of Biology, Colby College

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An improved protocol is presented for the measurement of transient gene expression from reporter constructs in barley aleurone cells after particle bombardment. The combination of automated grain grinding with 96-well plate enzyme assays provides high throughput for the procedure.

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Gene Expression Barley Aleurone Layers Particle Bombardment Reporter Constructs Plant Hormone Regulated Gene Expression Abscisic Acid Higher Throughput Gene Constructs Incubation Conditions Grace Lab Student Embryoless Grains Imbibing Solution Chloramphenicol Seed Coat Peeling Gold Microcarriers Vermiculite Plate Incubator
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