Journal
/
/
In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses
DOI:

07:26 min

December 05, 2019

, , , ,

Chapters

  • 00:05Title
  • 01:09Overview of Probe
  • 01:40Off-line Measurement
  • 03:43Particle Identification
  • 05:11On-Line Measurement
  • 06:03Results: Cumulative Single-cell Size Distribution of S. Cerevisiae
  • 06:39Conclusion

Summary

Automatic Translation

A photo-optical in situ microscopy device was developed to monitor the size of single cells directly in the cell suspension. The real-time measurement is conducted by coupling the photo-optical sterilizable probe to an automated image analysis. Morphological changes appear with dependence on the growth state and cultivation conditions.

Related Videos

Read Article