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Journal
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Immunology and Infection
/
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
DOI:
10.3791/60806-v
•
07:50 min
•
March 30, 2020
•
Dinesh Jaishankar
,
Cormac Cosgrove
,
Ryan J. Deaton
,
I. Caroline Le Poole
3
1
Robert Lurie Comprehensive Cancer Center and Department of Dermatology, Feinberg School of Medicine
,
Northwestern University
,
2
Department of Pathology
,
University of Illinois at Chicago
,
3
Department of Microbiology and Immunology, Feinberg School of Medicine
,
Northwestern University
Chapters
00:05
Introduction
00:39
Antibody and Fluorophore Selecting and Staining
01:52
Spectral Library Preparation
02:59
Multispectral Imaging
03:54
Spectral Unmixing
04:37
Analyzing Multispectral Images via Cell Segmentation and Phenotyping
05:52
Results: Representative Multispectral Imaging and Cell Quantification
07:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
We describe a rapid staining method to perform multispectral imaging on frozen tissues.
Tags
Multispectral Fluorescence Imaging
Biomarker Detection
Co-localization
Rapid Staining Method
Spectral Library
Machine Learning
Image Analysis
Article
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