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Whole-cell Recordings of Light Evoked Excitatory Synaptic Currents in the Retinal Slice
JoVE Journal
Biology
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JoVE Journal Biology
Whole-cell Recordings of Light Evoked Excitatory Synaptic Currents in the Retinal Slice
DOI:

19:33 min

July 02, 2008

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Chapters

  • 00:00Title
  • 00:10Preparations for the Dissection
  • 02:40Dissection of the Eye
  • 09:42Moving Slices to the Recording Chamber
  • 12:08Preparation of patch Electrodes
  • 13:21The Recording Rig
  • 14:09Whole-cell Recording
  • 17:40Recording Light-evoked Currents
  • 18:55Discussion/ Conclusion

Summary

Automatic Translation

This video shows the process of whole-cell voltage clamp recordings in the retinal slice of the aquatic tiger salamander. We demonstrate the preparation of the slice as well as how to perform patch clamp recordings during visual stimulation of the retina.

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