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 JoVE Biology

Protocolli per Microapplicator assistita Infezione delle larve di lepidotteri Baculovirus

1, 1, 1

1Department of Entomology, Iowa State University

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    Summary

    In questo video, dimostriamo microapplicator due tecniche utilizzate per infettare le larve di lepidotteri con baculovirus per determinare l'efficienza insetticida.

    Date Published: 8/23/2008, Issue 18; doi: 10.3791/889

    Cite this Article

    Li, H., Sparks, W., Bonning, B. Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus. J. Vis. Exp. (18), e889, doi:10.3791/889 (2008).

    Abstract

    Baculoviruses sono ampiamente utilizzati sia come vettori di espressione proteica e come agenti di controllo degli insetti infestanti. . Questo video mostra come le larve di lepidotteri possono essere infettati con tecniche microapplicator nell'intestino con poliedri baculovirus e nella emolinfa con il virus germogliato. Questo accompagnamento Springer protocolli sezione offre una panoramica del ciclo di vita baculovirus e l'uso di agenti baculoviruses come insetticida. Formulazione e applicazione di baculoviruses a fini di controllo dei parassiti sono descritte altrove.

    Protocol

    Si prega di visitare protocolli Springer per saperne di più sul baculovirus ingegneria come agente insecidical microapplicator e le tecniche utilizzate in questo saggio.

    Disclosures

    Comments

    2 Comments

    I would like to know who sells the syringe-aplicator like you show in this video Thank you MV Miranda
    Reply

    Posted by: AnonymousDecember 19, 2008, 8:54 AM

    The one used in these protocols was a Burkard Microprocessor Controller Pax-100.    I hope that will help you in your search.   W. O. Sparks
    Reply

    Posted by: AnonymousJanuary 29, 2009, 10:42 AM

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