1Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf,University of Hamburg, 2Department of Pediatric Surgery, University Medical Center Mannheim,University of Heidelberg, 3Division of Spine Surgery, Department of Trauma and Orthopedic Surgery,University Medical Center Hamburg-Eppendorf (UKE)
Neutrophil extracellular traps (NETs) are associated with various diseases, and immunofluorescence is often used for their visualization. However, there are various staining protocols, and, in many cases, only one type of tissue is examined. Here, we establish a generally applicable protocol for staining NETs in mouse and human tissue.
