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Immunology and Infection

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Staining and Imaging to Visualize Neutrophil Extracellular Traps

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ï»¿Autofluorescence Blocking and Antigen Retrieval in Tissue Samples (Video) | JoVE

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Immunology and Infection

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JoVE Journal Immunology and Infection

Autofluorescence Blocking and Antigen Retrieval in Tissue Samples

ï»¿Autofluorescence Blocking and Antigen Retrieval in Tissue Samples (Video) | JoVE

Staining and Imaging to Visualize Neutrophil Extracellular Traps

DOI:

10.3791/200336-v

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01:56 min

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August 18, 2023

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Lavinia Schoenfeld, Birgit Appl, Laia Pagerols-Raluy, Annika Heuer, Konrad Reinshagen, Michael Boettcher²

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf,University of Hamburg, ²Department of Pediatric Surgery, University Medical Center Mannheim,University of Heidelberg, ³Division of Spine Surgery, Department of Trauma and Orthopedic Surgery,University Medical Center Hamburg-Eppendorf (UKE)

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Keywords: Autofluorescence Blocking Antigen Retrieval Citrate Buffer Target Retrieval Solution TRS PH Deionized Water Heat Treatment Wet Chamber Autofluorescence-reducing Agent Ethanol Antigen Retrieval TRIS Buffered Saline Tween Hydrophobic Barrier Pen

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