Using Skin Biopsies to Generate Patient-Derived Podocytes

May 26, 2023

Generation of Patient-Derived Podocytes from Skin Biopsies (Video) | JoVE

02:02 min

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ï»¿Outgrowth of Dermal Fibroblasts from a Skin Punch Biopsy (Video) | JoVE

01:33 min

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Episomal Reprogramming of Dermal Fibroblasts to Generate Human-Induced Pluripotent Stem Cells (HiPSCs) (Video) | JoVE

03:26 min

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Selection, Expansion, and Quality Control of hiPSCs Generated from Dermal Fibroblasts (Video) | JoVE

02:41 min

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Victoria Rose, Janina Müller-Deile²

¹Department of Nephrology and Hypertension, University Hospital Erlangen,Friedrich-Alexander University (FAU) Erlangen-Nürnberg, ²Research Center on Rare Kidney Diseases (RECORD),University Hospital Erlangen

Summary

This manuscript describes a two-step protocol to generate patient-specific podocytes from dermal fibroblasts via episomal reprogramming into human-induced pluripotent stem cells (hiPSCs) and subsequent differentiation into podocytes.