Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove

De-Sialylated Monocyte-Derived-DCs from Isolated PBMCs Using Sialidase Treatment

October 20th, 2023
1,2, 1,2, 1,2,3, 4,5, 6, 1,2,7
1Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 3LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 4Department of Microbiology and Immunology, Albert Einstein College of Medicine, 5Department of Oncology, Albert Einstein College of Medicine, 6Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark, Zona Industrial da Gandra, 7CDG & Allies - Professionals and Patient Associations International Network (CDG & Allies - PPAIN), Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa

Summary
Automatic Translation

Un protocole unique et complet pour générer des cellules dendritiques dérivées de monocytes humains désialylés (mo-DCs) à partir de cellules mononucléées isolées du sang périphérique (PBMC) à l’aide d’un traitement à la sialidase est présenté. De plus, des méthodes permettant d’évaluer la caractérisation phénotypique et fonctionnelle des mo-DC et d’évaluer comment le traitement à la sialidase améliore le niveau de maturation des mo-DC sont décrites.

Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter