1Associate Laboratory i4HB – Institute for Health and Bioeconomy, NOVA School of Science and Technology,Universidade NOVA de Lisboa, 2UCIBIO – Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa, 3LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia,Universidade NOVA de Lisboa, 4Department of Microbiology and Immunology,Albert Einstein College of Medicine, 5Department of Oncology,Albert Einstein College of Medicine, 6Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark,Zona Industrial da Gandra, 7CDG & Allies – Professionals and Patient Associations International Network (CDG & Allies – PPAIN), Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa
En unik, omfattende protokol til generering af de-sialylerede humane monocytafledte dendritiske celler (mo-DC’er) fra isolerede mononukleære celler i perifert blod (PBMC’er) ved hjælp af en sialiidasebehandling præsenteres. Endvidere beskrives metoder til vurdering af fænotypisk og funktionel karakterisering af mo-DC’er og evaluering af, hvordan sialiidasebehandling forbedrer modningsniveauet for mo-DC’er.
