Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove

De-Sialylated Monocyte-Derived-DCs from Isolated PBMCs Using Sialidase Treatment

October 20th, 2023
1,2, 1,2, 1,2,3, 4,5, 6, 1,2,7
1Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 3LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 4Department of Microbiology and Immunology, Albert Einstein College of Medicine, 5Department of Oncology, Albert Einstein College of Medicine, 6Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark, Zona Industrial da Gandra, 7CDG & Allies - Professionals and Patient Associations International Network (CDG & Allies - PPAIN), Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa

Summary
Automatic Translation

En unik, omfattende protokoll for å generere de-sialylerte humane monocytavledede dendrittiske celler (mo-DCs) fra isolerte mononukleære celler i perifert blod (PBMC) ved bruk av en sialidasebehandling presenteres. Videre beskrives metoder for å vurdere fenotypisk og funksjonell karakterisering av mo-DCs og evaluere hvordan sialidasebehandling forbedrer modningsnivået til mo-DCs.

Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter