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Unveiling the Potential of Unpurified Recombinant AAVs in Cell Culture Research (Video) | JoVE
Production of Recombinant Adeno-Associated Viral Vectors and Transduction of Cultured Cells (Video) | JoVE
重组腺相关病毒(rAAV)广泛用于临床和临床前基因递送。rAAV的一个被低估的用途是培养细胞的稳健转导,无需纯化。对于刚接触rAAV的研究人员,我们提供了转基因盒克隆、粗载体生产和细胞培养转导的方案。
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