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check_url/65572?article_type=b
Crude Vector Production and Efficient DNA Delivery in Cell Cultures
October 20, 2023
check_url/65572?article_type=b
使用未纯化的重组腺相关病毒载体在培养细胞中进行转基因表达
01:49 min
•
check_url/65572?article_type=b
重组腺相关病毒载体的生产和培养细胞的转导
05:10 min
•
Brian Benyamini
,
Meagan N. Esbin
2
,
Oscar Whitney
,
Nike Walther
,
Anna C. Maurer
1
Department of Molecular and Cell Biology
,
University of California, Berkeley
,
2
Biophysics Graduate Group
,
University of California, Berkeley
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重组腺相关病毒(rAAV)广泛用于临床和临床前基因递送。rAAV的一个被低估的用途是培养细胞的稳健转导,无需纯化。对于刚接触rAAV的研究人员,我们提供了转基因盒克隆、粗载体生产和细胞培养转导的方案。
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