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Crude Vector Production and Efficient DNA Delivery in Cell Cultures

October 20th, 2023
1, 1,2, 1, 1, 1
1Department of Molecular and Cell Biology, University of California, Berkeley, 2Biophysics Graduate Group, University of California, Berkeley

Summary
Automatic Translation

Recombinant adeno-associated virus (rAAV) is widely used for clinical and preclinical gene delivery. An underappreciated use for rAAVs is the robust transduction of cultured cells without the need for purification. For researchers new to rAAV, we provide a protocol for transgene cassette cloning, crude vector production, and cell culture transduction.

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