Metabolite Profiling of Hard Coral Samples Using GC-MS

October 13, 2023

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02:41 min

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Coral Metabolism Quenching and Coral Tissue Removal from Coral Skeleton (Video) | JoVE

01:28 min

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Separation of Coral Host Tissues and Symbiodiniaceae Cells for Metabolite Extractions

02:02 min

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Preparation of Metabolites from a Coral Holobiont, Separated Coral Host Tissue, and Symbiodiniaceae Cells for Gas Chromatography-Mass Spectrometry Analysis

03:15 min

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Jennifer L. Matthews, Natasha Bartels, Sheik Nadeem Elahee Doomun, Simon K. Davy, David P. De Souza

¹Climate Change Cluster (C3),University of Technology Sydney, ²Metabolomics Australia, Bio21 Molecular Science and Biotechnology Institute,The University of Melbourne, ³School of Biological Sciences,Victoria University of Wellington

Summary

Here, we present the extraction and preparation of polar and semi-polar metabolites from a coral holobiont, as well as separated coral host tissue and Symbiodiniaceae cell fractions, for gas chromatography-mass spectrometry analysis.