علم الأحياء الجزيئي، العدد 102، BRCA2، سيرنا، خطوط الخلايا البشرية، إسكات الجينات، ترنسفكأيشن، immunoblotting Protocols and Video Articles

Research

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Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

Authors

Author

Loredana Moro¹, Nicoletta Guaragnella¹, Sergio Giannattasio¹

Institution

¹Institute of Biomembranes and Bioenergetics, National Research Council

Journal (Biology)

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Authors

Author

Sandy Chevrier¹, Romain Boidot¹

Institution

¹Department of Biology and Pathology of Tumors, Unit of Molecular Biology, Platform of Immunomonitoring and Genetics, Centre Georges-François Leclerc

Journal (Biology)

Quantitative Immunoblotting of Cell Lines as a Standard to Validate Immunofluorescence for Quantifying Biomarker Proteins in Routine Tissue Samples

Authors

Author

Alison M. Moore^1,2, Lee R. Boudreau³, Shakeel Virk³, David P. LeBrun^1,2

Institution

¹Department of Pathology and Molecular Medicine, Queen's University, ²Division of Cancer Biology and Genetics, Queen's Cancer Research Institute, ³Queen's Laboratory for Molecular Pathology, Department of Pathology and Molecular Medicine, Queen's University

Journal (Biology)

Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting

Authors

Author

Svetlana Konovalova¹

Institution

¹Research Programs Unit, Molecular Neurology, University of Helsinki

Journal (Biology)

SUMOylation Assay

An In Vitro Technique to Detect the SUMOylation Status of Substrate Proteins by Immunoblotting

SUMOylation Assay

An In Vitro Technique to Detect the SUMOylation Status of Substrate Proteins by Immunoblotting

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Education

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Targeted Cancer Therapies

JoVE Core (Core: 分子生物学)

Targeted Cancer Therapies

JoVE Core (Core: Cell Biology)

Loss of Tumor Suppressor Gene Functions

JoVE Core (Core: Cell Biology)

Combination Therapies and Personalized Medicine

JoVE Core (Core: 分子生物学)

Loss of Tumor Suppressor Gene Functions

JoVE Core (Core: 分子生物学)

Cancers Originate from Somatic Mutations in a Single Cell

JoVE Core (Core: Cell Biology)

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