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Cancer Research

hyperpolarized Published: December 30, 2016 doi: 10.3791/54751
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*1,2,3, *1, 1, 1,2, 1, 3,4, 1,5,6, 1, 2, 3, 3, 1
1Department of Nuclear Medicine, Klinikum rechts der Isar, Technische Universität München, 2Department of Chemistry, Technische Universität München, 3GE Global Research, 4Zentralinstitut für Medizintechnik der Technischen Universität München (IMETUM), Technische Universität München, 5Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, 6IDG Institute of Developmental Genetics, Helmholtz Zentrum München
* These authors contributed equally

Abstract

पिछले दशकों में, ट्यूमर मचान, restaging, उपचार की प्रतिक्रिया की निगरानी, और कैंसर की एक किस्म की पुनरावृत्ति का पता लगाने के लिए नए तरीकों 18 एफ fluorodeoxyglucose ([18 एफ के साथ राज्य के अत्याधुनिक पोजीट्रान एमिशन टोमोग्राफी के साथ संयोजन के रूप में उभरा है ] -FDG पीईटी)। 13 सी चुंबकीय अनुनाद इमेजिंग स्पेक्ट्रोस्कोपी (13 CMRSI) एक न्यूनतम इनवेसिव इमेजिंग तरीका है कि इन विवो में और वास्तविक समय में चयापचय की निगरानी में सक्षम बनाता है। 13 सी परमाणु चुंबकीय अनुनाद (एनएमआर) के आधार पर किसी अन्य विधि के साथ के रूप में, यह 13 सी और अपनी कम प्राकृतिक बहुतायत की अपेक्षाकृत कम gyromagnetic अनुपात के कारण कम थर्मल ध्रुवीकरण और बाद में एक कम संकेत करने वाली शोर अनुपात की चुनौती का सामना जैविक नमूने हैं। इन सीमाओं से पार पाते हुए, गतिशील परमाणु ध्रुवीकरण (DNP) बाद नमूना विघटन के साथ हाल ही में सामान्यतः सक्षम है एनएमआर और चुंबकीय अनुनाद इमेजिंग (एमआरआई) प्रणाली का इस्तेमाल किया मापने के लिएविभिन्न जैविक प्रणालियों में अध्ययन, और छवि कुंजी चयापचय मार्ग। एक विशेष रूप से दिलचस्प और होनहार 13 CMRSI में इस्तेमाल किया अणु [1- 13 सी] पाइरूवेट, सेलुलर ऊर्जा चयापचय जांच करने के लिए नैदानिक अध्ययन है, जो पिछले दस वर्षों में, व्यापक रूप से और अधिक हाल ही में इन विट्रो, preclinical, और, के लिए इस्तेमाल किया गया है कैंसर और अन्य बीमारियों में। इस अनुच्छेद में, हम एक 3.35 टी preclinical DNP hyperpolarizer का उपयोग कर विघटन DNP की तकनीक रूपरेखा और इन विट्रो अध्ययन में इसके उपयोग का प्रदर्शन। Hyperpolarization के लिए इसी तरह की एक प्रोटोकॉल के रूप में अच्छी तरह से vivo अध्ययन में सबसे अधिक भाग के लिए लागू किया जा सकता है। ऐसा करने के लिए, हम लैक्टेट डिहाइड्रोजनेज (LDH) का इस्तेमाल किया और [1- 13 सी] एक प्रोस्टेट कार्सिनोमा सेल लाइन में लैक्टेट, PC3, इन विट्रो 13 CMRSI उपयोग करने के लिए [1- 13 सी] पाइरूवेट के चयापचय प्रतिक्रिया उत्प्रेरित।

Materials

Name Company Catalog Number Comments
HyperSense DNP Polariser Oxford Instruments 3.35 T preclinical DNP hyperpolarizer
GE/Agilent MR901 GE Healthcare/Agilent Technologies 7 T preclinical MRI scanner, with small bore designed for experiments onrodent
Spinsolve Carbon Magritek 1 T NMR spectrometer with permanent magnet
Deuterium Oxide Sigma Aldrich 7789-20-0
Sodium phosphate dibasic Sigma Aldrich 7558-79-4
Sodium phosphate monbasic Sigma Aldrich 7558-80-7
Sodium hydroxide Sigma Aldrich 1310-73-2
Disodium edetate Sigma Aldrich 6381-92-6
Pyruvic acid - 13C1 Cambridge Isotopes Laboratories CLM-8077-1
Dotarem (0.5 mmol/L) Guerbet gadoterate meglumine  
tris (8-carboxy-2,2,6,6-tetra-(hydroxyethyl)-benzo-[1,2–4,5]-bis-(1,3)-dithiole-4-yl)-methyl sodium salt (OX063) GE Healthcare trityl radical used as a sourse of free electron
PC3 cell line ATCC CRL1435
F-12K medium  ATCC 30-2004
Fetal Bovine Serum ATCC SCRR-30-2020
Trypsine-EDTA Solution, 1x ATCC 30-2101
Sample plastic cup Oxford Instruments
Trypan blue Bio-Rad 145-0013-MSDS

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hyperpolarized<sup&gt; 13</sup&gt; सी मेटाबोलिक चुंबकीय अनुनाद स्पेक्ट्रोस्कोपी और इमेजिंग
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Kubala, E., Muñoz-Álvarez, … More

Kubala, E., Muñoz-Álvarez, K. A., Topping, G., Hundshammer, C., Feuerecker, B., Gómez, P. A., Pariani, G., Schilling, F., Glaser, S. J., Schulte, R. F., Menzel, M. I., Schwaiger, M. Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging. J. Vis. Exp. (118), e54751, doi:10.3791/54751 (2016).

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