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Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
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生物学
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JoVE 杂志 生物学
Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

DOI:
10.3791/2053-v

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13:20 min

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July 29, 2010

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, ,
1Department of Biology,University of Virginia

Chapters

  • 00:05Title
  • 01:11Introduction
  • 01:33Crosslinking
  • 03:32Preparing Chromatin
  • 05:37Chromatin IP (ChIP)
  • 07:50Collecting Immunocomplexes
  • 10:01Recovering the DNA
  • 11:45Representative Results
  • 12:44Conclusion

Summary

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This protocol describes how chromatin immunoprecipitation (ChIP) is used to study the dynamic alterations to the chromatin template that regulate transcription induced by a signal transduction pathway.

Tags

Chromatin ImmunoprecipitationChIPHistone ModificationActivated Gene ExpressionHuman CellsSTAT Transcription FactorsExtracellular LigandsPhosphorylationTyrosine ResidueNucleusTarget GenesGrowthDifferentiationHomeostasisImmune ResponseDysregulationHuman DiseaseCancersAutoimmune DiseasesChromatin TemplateATP-dependent ComplexesCovalent Histone ModificationsDNA Methylation

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