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Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
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Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

Article DOI: 10.3791/2053-v • 13:21 min • July 29th, 2010
July 29th, 2010 •
1, 1, 1
1Department of Biology, University of Virginia

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This protocol describes how chromatin immunoprecipitation (ChIP) is used to study the dynamic alterations to the chromatin template that regulate transcription induced by a signal transduction pathway.

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Chromatin Immunoprecipitation ChIP Histone Modification Activated Gene Expression Human Cells STAT Transcription Factors Extracellular Ligands Phosphorylation Tyrosine Residue Nucleus Target Genes Growth Differentiation Homeostasis Immune Response Dysregulation Human Disease Cancers Autoimmune Diseases Chromatin Template ATP-dependent Complexes Covalent Histone Modifications DNA Methylation
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